A rapid and efficient one-tube PCR-based mutagenesis technique usingPfuDNA polymerase
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چکیده
منابع مشابه
A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase
A rapid method for efficiently generating site-directed mutations on a clean sequence background is described. This modification of the megaprimer PCR mutagenesis approach can be performed in one tube in less than 4.5 hours, and does not require purification of intermediate products. High fidelity of DNA sequence replication is obtained by employing Pfu DNA polymerase and limiting the total num...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.13.2587